In the best of all possible worlds, the water in the bag would be the same as the water in the tank, or failing that, you would have enough time, patience and the facilities to slowly adjust the water in the bag (a large 10 gallon bag, let's say - with filter and heater) to equal the water values in your target tank. :) In the real world, I've used the two following methods to acclimate fish: 1) where the water values in the tank are similar to the water values in the bag, then I acclimate in the typical fashion, i.e., equalize water temperatures in bag and tank, add tank water slowly to the bag (1/4 of the volume of the bag every 15 minutes or so), net fish from bag and place fish in tank. 2) where the water values are very dissimilar, I do something similar to Michael (with or without Amquel - which is used primarily to offset the higher pH/ammonia effect - depending upon pH of tank water), which is to net and dump without intermediary acclimation. The theory is that while the first method is preferable, when acclimating fish to very different water values, a one time acclimation is less stressful than rapid acclimation to multiple intermediate water values. In other words (remember, pH is logarithmic) a jump from 5.5 to 7.5 is stressful, but not as stressful as acclimating from 5.5 to 6.5 and then shortly thereafter from 6.5 to 7.5 (watch the ammonia!). Of course, too big of a jump and you kill the fish... yep... it's not always easy.... -mk ------------------------------------------------------------------------- This is the apistogramma mailing list, apisto@listbox.com. For instructions on how to subscribe or unsubscribe or get help, email apisto-request@listbox.com. Search http://altavista.digital.com for "Apistogramma Mailing List Archives"!