I do inject CO2 into the tanks that Erik mentioned, but only into the top tank. The pH seems to stay pretty constant all the way down when I do that. One thing to be aware of with oyster shells and cuttle bone is that they are primarily calcium carbonate (CaCO3). They can be a nice addition to a tank to help buffer the pH and add trace minerals that shrimp and some other animals really like. The CaCO3 will only dissolve enough until the pH raises to a certain point then it will stop adding hardness to the water for the most part. You have to be careful though when you start injecting CO2, especially if it's on a pH regulator. If the regulator is trying to drive too low of a pH level with CO2 you can end up creating a cycle where the CaCO3 will balance the added CO2 to the point that the water is extremely hard with toxic CO2 levels for your fish. Jesse -----Original Message----- From: gsas-member-bounces@thekrib.com [mailto:gsas-member-bounces@thekrib.com] On Behalf Of Erik Olson Sent: Monday, October 19, 2009 1:47 PM To: Greater Seattle Aquarium Society member chat Subject: Re: [GSAS-Member] CO2 Question > And such a thing could very well work on an existing system like Jesse's > (cf. 2009 Home show -- http://www.gsas.org/homeshow2009.torrent) where he > has three cascading tanks on top of each other. > > - Erik _______________________________________________ GSAS-Member mailing list GSAS-Member@thekrib.com http://lists.thekrib.com/mailman/listinfo/gsas-member