Roy, I tried your suggestion as I am using API's Nitrate test solution that is part of their master test kit (the kind with two bottles). I did another test last night with your recommendation of plenty of vigorous shaking. No such luck. Nitrate is still reading 0. Also, when I went this morning to give my fish breakfast before I left for work, one of my oto's had was lying on the bottom, upside down, deceased. The other appeared to be fine and the danios are all just as active as always. Does anyone know about a way to tell if the tank is fully cycled w/o using ammonia/nitrite/nitrate levels to verify? Meg's suggestion (below) sounds like a possible explanation but with the death of a hearty fish like an oto I'm a bit concerned now and hesitant to introduce more less-hearty fish without knowing the water is definitively safe. Any suggestions? -M- ----------------- From Meg ----------------- Perhaps the 0 nitrate reading is due to your plants using it up? If there are only a handful of fish compared to the amount of water, you might have a small concentration of nitrate that the plants are able to keep up with. _______________________________________________ GSAS-Member mailing list GSAS-Member@thekrib.com http://lists.thekrib.com/mailman/listinfo/gsas-member