>Can it be regulated >to maintain a desired level once it is there? TIA Why mess with the DIY yeast for CO2? The best place to get info on CO2 addition is Aquatic-Plants-Owner@actwin.com. This is the planted tank people and most of us use CO2 addition as fertilizer and or to lower the pH. I personally would rather spend the time growing fruit flies or working on my tanks than messing with the yeast system. I use bottled CO2. Do you just bubble it into the tank or do you do something to make it dissolve easier? I put my through Lees air diffusers (cheap) that generate very fine bubbles. I also have a needle valve that I use to contol the flow. I also used some clear 1/2" flexible tubing and made a loop. I filled it partially with water and count the bubbles to get an idea of the flow rate and make the necessary adjustments to keep the pH around 6.4. I know some people have had to put in a bypass valve to relieve the excess capacity of their generators. Check out the Krib for more info