> Andrew > I chose leaves that were attached to branches that had fallen off the tree for whatever reason...I then boiled the leaves in a large pot for ten minutes... Here's where I would like some suggestions. I'm going to put the water that was boiled with the leaves into the new tank for the fish. I had boiled peat at one point and had good results from the water off of the peat. Also, I wanted to be sure that any organisms on the leaves was killed, fungus and bacteria in particular... >> Erik >> Boiling (Kathy says a 20 minute boil kills all bacteria/micro-orgs), straining, and dumping the leaves (sans the water) in the tank. The leaves continue, btw, to leach tannins into the water, so the new tank we put them in now has a very dark rich tea color and a pH of 5.8... I've used a variety of oak leaves over the years as they're one of my favorite sources of tannins. Oaks are also one of the richer sources of tannins available. Kathy's suggestion of a 20 minute boil follows about the same usage guidelines for autoclaves and such, so I would assume it to be sufficient time to achieve sterility. In all honesty, though, I've never considered sterility for a couple of reasons, chief among them being that I tend to boil them for 30 minutes in order to ensure they're waterlogged and to get the heaviest portion of the tannins out. I've never found the boil water to be unfit for subsequent use, either - at least not to memory. But because of the high tannin content, you are prudent in watching the amounts mixed. If you are used to using boiled peat extracts this should present no problem, and probably comes automatically. Any left- over boil- off of mine is usually added to my peat aging barrel, along with the remaining leaves and some of the bark and small branches. The aging barrel tends to collect all manner of odds and ends in my own personal efforts to get as many different humics into the mix as possible rather than concentrating on any particular one. I must confess to not having kept any of the extremely "delicate" Apistos (that always seemed an oxymoron to me, as I have difficulty picturing anything that can survive in the wild as "delicate" - just extremely "fussy" about the conditions it needs for robust health). But some of the other fry I've raised take every bit of the effort that Apistos do - getting Cardinal Tetras to breed is the easy part, raising a successful brood is far from it. To date, I've not lost so much as a grass shrimp that I can attribute in any way to the use of oak. -Y- David A. Youngker http://www.mindspring.com/~nestor10 nestor10@mindspring.com ------------------------------------------------------------------------- This is the apistogramma mailing list, apisto@majordomo.pobox.com. For instructions on how to subscribe or unsubscribe or get help, email apisto-request@majordomo.pobox.com. Search http://altavista.digital.com for "Apistogramma Mailing List Archives"!