Hi Bob, I am still pretty new at raising daphnia myself but I will try to do my best to answer your question. > Can you briefly summarize how you are raising your daphnia? The best people to ask about raising Daphnia are the members of Live-Foods mailing list. FINS site has the searchable archives of their messages. It has helped me a lot so far. The members are great at giving you the tips you need quickly. > the first time they didn't die, but they didn't multiply > either. The second time they expired quickly. Do you think that the batch that did not multiply might have been underfed or overcrowded? I was raising them in old > aquarium water in an unused fish box outdoors in partial shade (I live > in San Jose, CA,USA). What temp range can they tolerate? As far as temperature for daphnia is concerned, some people say that they do not tolerate high temperature. However, LFS Cultures told me that they raise theirs (D. magna and D. pulex) outside in the heat of the summer in Mississippi without any problem. The members of Japanese Apistogramma mailing list told me that they collect daphnia from rice paddies and keep them going all summer long outside. A few people on the Live-Foods mailing list have the Japanese species of daphnia called Moina which supposedly tolerate heat better. Maybe they can direct you to the source. Good luck, Tomoko ------------------------------------------------------------------------- This is the apistogramma mailing list, apisto@listbox.com. For instructions on how to subscribe or unsubscribe or get help, email apisto-request@listbox.com. Search http://www.digital.com for "Apistogramma Mailing List Archives"!